Sport Biosciences

The effect of high-intensity interval training (HIIT) on the content of proteins upstream of the initiation of the autophagy pathway in the EDL skeletal muscle of rats with type 2 diabetes

Articles in Press

Document Type : Research Paper

Authors

Arman Rastegari
Abbas Ali Gaeini
Mohammad Reza Kordi
Ali Asghar Ravasi
Reza Nouri

Department of sport physiology, Faculty of Sport Sciences and Health, University of Tehran, Tehran, Iran

10.22059/jsb.2025.387759.1668
Abstract
Introduction: Proteins of the AMPK and mTOR pathways are key factors in regulating mitophagy and autophagy mechanisms, which exercise training can affect in regulating or disrupting; Therefore, the aim of this study was to investigate the effect of high-intensity interval training (HIIT) on the content of proteins upstream of the initiation of the autophagy pathway in the EDL skeletal muscle of rats with type 2 diabetes.

Methods: In this experimental study, 18 male Sprague-Dawley rats with an average weight of 320±30 g were purchased. 12-rats were induced with type 2 diabetes by injection of nicotinamide and streptozotocin solutions. Diabetic rats were randomly divided into 2 HIIT and diabetic control groups; A healthy control group was also included. The HIIT program consisted of 5 sessions 4-minute bouts with an intensity of 85 to 95% of maximum speed. One-way ANOVA and Tukey post-hoc tests were used in SPSS version 29 software to analyze the data.

Results: AMPK and TSC1/2 proteins content showed a significant increase after eight weeks of HIIT (P<0.05). This increase was greater in the HIIT groups than in the diabetic and healthy control groups (P<0.05). In contrast, no significant difference was observed in the content of Rheb and mTOR proteins (P>0.05).

Conclusion: The increase in AMPK and TSC1/2 proteins and the lack of significant changes in the content of Rheb and mTOR proteins could be consistent with the activation of the AMPK pathway, the inactivation of the mTOR pathway, and the initiation of the downstream autophagy and mitophagy pathways.

Keywords

High-Intensity Interval Training
TSC1/2 Protein
Rehb Protein
Type 2 Diabetes
Subjects
Sport Biosciences

Articles in Press, Accepted Manuscript
Available Online from 27 May 2025

  • Receive Date 12 April 2025
  • Revise Date 26 May 2025
  • Accept Date 27 May 2025

Home

Contact Us