The aim of this study was to investigate the acute effects of two resistance exercise protocols with slow and fast movements on some anabolic and catabolic hormones. Hence, in a randomized crossover design, 10 young men (age: 23.3±1.76 yr) were divided into 3 groups: control (Con), resistance exercise with slow movement (SM), and resistance exercise with fast movement (FM). Subjects performed 6 movements in 4 sets at 60–65% of 1RM. Blood sampling were taken before, immediately and 30 min. after the exercise. The results showed that both SM and FM significantly increased GH immediately after the exercise (250% and 200% respectively), but there was no difference between SM and FM. In SM group, testosterone significantly increased immediately after the exercise. SM and FM had no effect on cortisol, insulin, and glucose. It can be concluded that SM has more effect on testosterone secretion compared with FM.