Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license
Authors
1 Ph.D student, Exercise physiology, Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, University of Tabriz, Tabriz, Iran
2 Assistant Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tabriz, Tabriz, Iran
3 Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, University of Tabriz, Tabriz, Iran.
4 Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Abstract
Introduction: This study aimed to investigate the effect of eight weeks of aerobic training on miR-30c expression in heart tissue and glycemic indices of T2DM rats.
methods: 20 male Wistar rats (239±28 g and 8-6 weeks old) were randomly divided into four groups (n=5) Diabetic Control, Healthy Control, Diabetic Training, and Healthy training. A number of 10 rats were fed with 60% high-fat food and then subjected to Streptozotocin symptoms. The training groups were placed in an aerobic training program on a treadmill (with a speed of 5-24 m/min) for eight weeks. Glucose was measured by glucose method using fasting blood insulin through an ELISA kit and to check miR-30c gene expression by Real-time PCR method. Data analysis was done with a one-way analysis of variance and Tukey's interpretation at a significance level of P≥0.05.
Results: Eight weeks of T2DM led to a significant increase in glucose, insulin, and insulin resistance (P=0.001). Eight weeks of aerobic training led to a significant decrease in glucose levels and insulin resistance (P=0.001). Also, having diabetes led to a significant decrease in miR-30c gene expression compared to the healthy control group (P=0.023). Although this reduction was somewhat increased with eight weeks of aerobic training compared to the diabetic control group, this increase was not significant (P<0.05).
Conclusion: It seems that the duration or intensity of training applied in the present study is still insufficient to increase the expression levels of the miR-30c gene, which requires more studies in this field.
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