Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license
Authors
- Majid Seifi Azar Nezhad 1
- Roghayeh Pouzesh Jadidi 2
- Karim Azali Alamdari 3
- Jabbar Bashiri 4
- Masoud Asgharpour-Arshad 5
1 1- Department of Sport Sciences, Faculty of Educational Sciences and Psychology, Tabriz branch, Islamic Azad University, Tabriz, Iran
2 Department of Exercise Physiology, Tabriz branch, Islamic Azad University, Tabriz, Iran
3 Department of Sport Sciences, Faculty of Psychology and Educational Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran.
4 4- Department of Sport Sciences, Faculty of Educational Sciences and Psychology, Tabriz branch, Islamic Azad University, Tabriz, Iran
5 Department of Physical Education and Sport Sciences, Faculty of Basic Sciences, Amin Police University, Tehran, Iran
Abstract
Introduction: The aim of this research was to investigate the effect of 8 weeks of aerobic training and curcumin supplementation on mitochondrial Drp1 and MFN2 genes expression in cardiomyocytes of male myocardial infarction (MI) model rats.
Methods: In the present experimental study, 32 male rats were randomly divided into four groups of control (MI), training (MI), curcumin (MI), and Concomitant (MI) following intraperitoneal injection of isoproterenol (100 mg/kg) for two consecutive days to induce myocardial infarction. Aerobic exercise was performed for eight weeks of moderate-intensity running on a treadmill, and Curcumin was administrated through oral gavage 15 mg/kg a day. At the end of the intervention, Drp1 and MFN2 genes expression in cardiomyocytes was measured by Real-time PCR method. The data were analyzed with the Shapiro-Wilk test and analysis of variance. Results: In training (MI), curcumin (MI), and Concomitant (MI) groups, Drp1 gene expression was lower and MFN2 gene expression was higher than the control (MI) group (p<0.05 in all cases). In the Concomitant (MI) group, compared to both curcumin (MI) and training (MI) groups, the effect value on both variables MFN2 (p=0.006 and p=0.019) and DRP1 (p=0.006 and p=0.011) p) was more. Conclusion: Aerobic training and curcumin consumption can probably increase the fusion and decrease the fission of mitochondria in cardiomyocytes of male myocardial infarction model rats. However, due to research limitations, further investigation is still needed.
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