Sport Biosciences

Interactive Effect of Resistance Training and Pumpkin Seed Consumption on the Levels of Inhibitory (Glutathione and ATP) and Destructive (Cytochrome-C and Malon di Aldehyde) Factors in Lung Tissue of Rats Poisoned With Hydrogen Peroxide

Document Type : Research Paper

Authors

Nazafarin Mohammad 1
Hasan Matinhomaee 2
Seyed Ali Hoseini 3

1 Department of Sports Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.

2 Corresponding Author, Department of Sports Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.

3 Department of Sports Physiology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran.

https://doi.org/10.22059/jsb.2022.343665.1534
Abstract
Introduction: Pulmonary poisoning and the production of Reactive Oxygen Species (ROS) lead to impaired respiratory gas exchange and cause DNA damage. Pumpkin seeds with powerful antioxidant properties can reduce ROS production. Therefore, the present study aimed to investigate the interactive effect of resistance training and pumpkin seed consumption on the levels of inhibitory (Glutathione and ATP) and destructive (Cytochrome-C and Malondialdehyde) factors in lung tissue of rats poisoned with hydrogen peroxide.
Methods: forty-two male Wistar rats with an approximate weight of 250±50 kg were selected and randomly divided into seven groups (six rats in each group) including 1. Healthy Control, 2. Poisoned Control, 3. Resistance Training, 4. One mg/kg Pumpkin Seeds, 5. Two mg/kg Pumpkin Seeds, 6. Resistance Training + One mg/kg Pumpkin Seeds, and 7. Resistance Training + Two mg/kg Pumpkin Seeds. The resistance training protocol consisted of climbing a one-meter ladder with a 2-centimeter distance between each step and an 85-degree incline. Data were analyzed using two-way ANOVA and Bonferroni post hoc test in SPSS software at a significance level of α=0.05.
Results: Resistance training combined with the consumption of pumpkin seeds led to a significant effect on ATP, Cytochrome-C, GSH, MDA, and PAB concentrations in lung tissue (P=0.001).
Conclusion: The interaction of performing resistance training with pumpkin seed consumption can reduce the production of ROS via toxins such as H2O2 in lung tissue, by increasing factors such as ATP and GSH concentration as well as decreasing Cytochrome-C, MDA, and PAB concentrations.

Keywords

cytochrome-C
hydrogen Peroxide
halondialdehyde
pumpkin seed
resistance training
Subjects
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Sport Biosciences
Volume 14, Issue 3
Autumn 2022
Pages 65-79

  • Receive Date 27 May 2022
  • Revise Date 29 August 2022
  • Accept Date 06 November 2022

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