Sport Biosciences

The Effect of Six Weeks of High-Intensity Interval Training (HIIT) and Endurance on Blood Glucose and Follistatin Protein Content in the left Ventricular Tissue of the Heart of Male Rats with type 1 Diabetes

Document Type : Research Paper

Authors

Hajar Khanjani 1
Mohammedreza Esmaelzadeh Toloee 2

1 Master of Exercise Physiology, Sport Sciences Faculty, Shomal University, Amol, Iran

2 Assistant Professor. Department of Exercise Physiology, Sport Sciences Faculty, Shomal University, Amol, Iran.

https://doi.org/10.22059/jsb.2021.324379.1471
Abstract
Follistatin is a key protein in the regulation of muscles (heart and Skeletal) hypertrophy that type 1 diabetes can lead to dysfunction. Therefore, the aim of the present study was to investigate the effect of six weeks of high-intensity interval training (HIIT) and endurance on blood glucose and Follistatin protein content in the left ventricular tissue of the heart of male rats with type 1 diabetes. In this study, 18 head 2 Sprague-Dawley male rats with a mean weight of 300±20 g were selected. After becoming type 1 diabetic through streptozotocin solution, they were randomly divided into 3 groups: 1) HIIT training 2) endurance training and 3) control (6 heads per group). Exercise groups exercised 4 days a week for 6 weeks according to HIIT and endurance training programs. One-way ANOVA and Tukey post hoc test were used to analyze the data. Six weeks of HIIT and endurance training resulted in a significant decrease in blood glucose levels (p=0.0001) and a significant increase in Follistatin protein content (p=0.0001) compared to the control group. HIIT and endurance training lowered blood glucose levels, indicating that these two types of training could be appropriate and non-invasive treatment for control diabetes. Also, endurance training and HIIT training increased the intracellular content of Follistatin protein, which can be useful for physiological hypertrophy and reduction of cardiac pathological complications, including diabetic cardiomyopathy.

Keywords

Blood Glucose
Diabetes
Endurance Training
Follistatin. Heart
High-Intensity Interval Training
Subjects
  1. Yang, J.-S., et al., Autophagy and its link to type II diabetes mellitus. Biomedicine, 2017. 7(2).
  2. DiMeglio, L.A., C. Evans-Molina, and R.A. Oram, Type 1 diabetes. The Lancet, 2018. 391(10138): p. 2449-2462.
  3. Konduracka, E., et al., Myocardial dysfunction and chronic heart failure in patients with long-lasting type 1 diabetes: a 7-year prospective cohort study. Acta diabetologica, 2013. 50(4): p. 597-606.
  4. Gilbert, R.E. and H. Krum, Heart failure in diabetes: effects of anti-hyperglycaemic drug therapy. The Lancet, 2015. 385(9982): p. 2107-2117.
  5. Miki, T., et al., Diabetic cardiomyopathy: pathophysiology and clinical features. Heart failure reviews, 2013. 18(2): p. 149-166.
  6. Chavali, V., S.C. Tyagi, and P.K. Mishra, Predictors and prevention of diabetic cardiomyopathy. Diabetes, metabolic syndrome and obesity: targets and therapy, 2013. 6: p. 151.
  7. Huynh, K., et al., Diabetic cardiomyopathy: mechanisms and new treatment strategies targeting antioxidant signaling pathways. Pharmacology & therapeutics, 2014. 142(3): p. 375-415.
  8. Boudina, S. and E.D. Abel, Diabetic cardiomyopathy, causes and effects. Reviews in Endocrine and Metabolic Disorders, 2010. 11(1): p. 31-39.
  9. Egan, B. and J.R. Zierath, Exercise metabolism and the molecular regulation of skeletal muscle adaptation. Cell metabolism, 2013. 17(2): p. 162-184.
  10. Murton, A. and P. Greenhaff, Resistance exercise and the mechanisms of muscle mass regulation in humans: acute effects on muscle protein turnover and the gaps in our understanding of chronic resistance exercise training adaptation. The international journal of biochemistry & cell biology, 2013. 45(10): p. 2209-2214.
  11. Hansen, J.S., et al., Circulating follistatin is liver-derived and regulated by the glucagon-to-insulin ratio. The Journal of Clinical Endocrinology & Metabolism, 2016. 101(2): p. 550-560.
  12. Castonguay, R., et al., Follistatin-288-Fc fusion protein promotes localized growth of skeletal muscle. Journal of Pharmacology and Experimental Therapeutics, 2019. 368(3): p. 435-445.
  13. Jensky, N.E., et al., Exercise does not influence myostatin and follistatin mRNA expression in young women. Journal of strength and conditioning research/National Strength & Conditioning Association, 2010. 24(2): p. 522.
  14. Giallauria, F., et al., Exercise training early after acute myocardial infarction reduces stress-induced hypoperfusion and improves left ventricular function. European journal of nuclear medicine and molecular imaging, 2013. 40(3): p. 315-324.
  15. Tao, L., et al., Exercise for the heart: signaling pathways. Oncotarget, 2015. 6(25): p. 20773.
  16. Khoramshahi, S., Effect of five weeks of high-intensity interval training on the expression of miR-23a and Atrogin-1 in gastrocnemius muscles of diabetic male rats. Iranian Journal of Endocrinology and Metabolism, 2017. 18(5): p. 361-367.
  17. Cassidy, S., et al., High-intensity interval training: a review of its impact on glucose control and cardiometabolic health. Diabetologia, 2017. 60(1): p. 7-23.
  18. Gibala, M.J., et al., Physiological adaptations to low‐volume, high‐intensity interval training in health and disease. The Journal of physiology, 2012. 590(5): p. 1077-1084.
  19. Trilk, J.L., et al., Effect of sprint interval training on circulatory function during exercise in sedentary, overweight/obese women. European journal of applied physiology, 2011. 111(8): p. 1591-1597.
  20. Biglari, S., et al., The effect of 8 weeks high-intensity interval training on myostatin and follistatin gene expression in gastrocnemius muscle of the rats. Journal of Arak University of Medical Sciences, 2018. 21(1): p. 1-10.
  21. Rashidlamir, A., et al., The effect of eight weeks resistance and aerobic training on myostatin and follistatin expression in cardiac muscle of rats. Journal of cardiovascular and thoracic research, 2016. 8(4): p. 164.
  22. Aghaei, N., et al., the effect of 4 weeks’aerobic training on the content of mtorc1 signaling pathway proteins in heart tissue of type 1 diabetes rats. Iranian Journal of Diabetes and Metabolism, 2019. 18(3): p. 116-125.
  23. Jokar, M., M. Sherafati Moghadam, and M. Salesi, The effect of endurance exercise on the content of ampk and pgc-1α proteins in the left ventricular heart tissue of rats with type 2 diabetes. Iranian Journal of Diabetes and Metabolism, 2020. 19(5): p. 252-260.
  24. Jokar, M. and M. Sherafati Moghadam, High intensity interval training inhibits autophagy in the heart tissue of type 2 diabetic rats by decreasing the content of FOXO3a and Beclin-1 proteins. Iranian Journal of Diabetes and Metabolism, 2019. 18(6): p. 292-299.
  25. Garcia, N.F., et al., Metabolic parameters and responsiveness of isolated iliac artery in LDLr-/-mice: role of aerobic exercise training. American journal of cardiovascular disease, 2017. 7(2): p. 64.
  26. Zarei, F., et al., the effects of 4 weeks high intensity interval training on mammalian rapamycin target protein (mtor) and sterol transcription factor regulatory protein-1 (srebp1) proteins content in diabetics obese rats adipose tissue. Iranian Journal of Diabetes and Metabolism, 2019. 19(1): p. 26-35.
  27. Shabani, M., M. Sherafati Moghadam, and K. Moghaddami, The effect of endurance training on protein kinase-b and mechanical target of rapamycin in the left ventricle of the heart of diabetic rats induced by streptozotocin and nicotinamide. Iranian Journal of Diabetes and Metabolism, 2020. 19(6): p. 309-317.
  28. Little, J.P., et al., Effects of high-intensity interval exercise versus continuous moderate-intensity exercise on postprandial glycemic control assessed by continuous glucose monitoring in obese adults. Applied physiology, nutrition, and metabolism, 2014. 39(7): p. 835-841.
  29. Hafstad, A.D., et al., High-and moderate-intensity training normalizes ventricular function and mechanoenergetics in mice with diet-induced obesity. Diabetes, 2013. 62(7): p. 2287-2294.
  30. Mohammadi, E. and F. Nikseresht, Effect of 8 Weeks of Incremental Endurance Training on Antioxidant Enzymes and Total Antioxidant Status of Cardiac Tissue in Experimental Diabetic Rats. Journal of Shahid Sadoughi University of Medical Sciences, 2020.
  31. Fereshtian, S., et al., Comparison of the effects of two types of high intensity interval training on the gene expression of collagen 1, 2, and SMAD/3 in the left ventricle of male rats in type 2 diabetes induced by streptozotocin. Daneshvar Medicine: Basic and Clinical Research Journal, 2020. 28(3): p. 42-54.
  32. Golshan, H., et al., Effect of Different HIIT Protocols on the Glycemic Control and Lipids Profile in Men with type 2 diabetes: A Randomize Control Trial. Iranian journal of diabetes and obesity, 2020.
  33. Codella, R., I. Terruzzi, and L. Luzi, Why should people with type 1 diabetes exercise regularly? Acta diabetologica, 2017. 54(7): p. 615-630.
  34. Chiang, J.L., et al., Type 1 diabetes through the life span: a position statement of the American Diabetes Association. Diabetes care, 2014. 37(7): p. 2034-2054.
  35. Colberg, S.R., et al., Physical activity/exercise and diabetes: a position statement of the American Diabetes Association. Diabetes care, 2016. 39(11): p. 2065-2079.
  36. Arabzadeh, E., et al., Alteration of follistatin-like 1, neuron-derived neurotrophic factor, and vascular endothelial growth factor in diabetic cardiac muscle after moderate-intensity aerobic exercise with insulin. Sport Sciences for Health, 2020: p. 1-9.
  37. Elliott, B.T., et al., Lifelong exercise, but not short‐term high‐intensity interval training, increases GDF 11, a marker of successful aging: a preliminary investigation. Physiological reports, 2017. 5(13): p. e13343.
  38. Francois, M.E. and J.P. Little, Effectiveness and safety of high-intensity interval training in patients with type 2 diabetes. Diabetes Spectrum, 2015. 28(1): p. 39-44.

 

Sport Biosciences
Volume 13, Issue 3
Autumn 2021
Pages 351-365

  • Receive Date 03 June 2021
  • Revise Date 17 November 2021
  • Accept Date 05 October 2021

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