Sport Biosciences

The Effect of 10 Weeks of Exhaustive Swimming on Gene Expression of Histone Deacetylase-4 and Myocyte Enhancer Factor-2c in Left Ventricle in Male Rats

Document Type : Research Paper

Authors

Parivash Piraki 1
Ahmad Hematfar 2
Naser Behpour 3
Mohamad Ali Samavati Sharif 4

1 Sport Physiology Student, Department of Physical Education, Boroujerd Branch, Islamic Azad University, Boroujerd, Iran

2 Assistant Professor in Sport Physiology, Department of Physical Education, Boroujerd Branch, Islamic Azad University, Boroujerd, Ira

3 Associate Professor in Sport Physiology, Department of Physical Education,Boroujerd Branch,Islamic Azad University,Boroujerd,Iran

4 Associate Professor in Sport Physiology,Sport Science Faculty,Buali Sina University,Hamadan,Iran

https://doi.org/10.22059/jsb.2018.243078.1213
Abstract
Epigenetic changes and cardiac reconstruction are among the most important adaptations in endurance training. The aim of this study was to investigate the gene expression of histone deacetylase-4 and myocyte enhancer factor-2c (MEF-2c) in male rats in interaction with exhaustive swimming. 12 male Wistar rats (mean age: 7±1 weeks, weight 275±25 gr) were randomly divided into two groups (each group 6 subjects): control and training. After performing the exhaustive swimming for 3 hours per session and 5 days per week for 10 weeks, their left ventricle was isolated; then the gene expression were investigated by Real Time-PCR. Data were analyzed by independent t test and the significance level was P≤0.05. The results showed that after 10 weeks of exhaustive swimming, the HDAC4 gene significantly increased (P=0.02), but MEF-2c gene significantly decreased (P=0.001) compared with the control group. Therefore, 10 weeks of exhaustive swimming increased gene expression of HDAC-4 and consequently reduced gene expression of MEF-2c by an increase in the activity of transcription factors attached to HDAC-4.
 

Keywords

Exhaustive swimming
histone deacetylase-4
myocyte enhancer factor-2c
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Sport Biosciences
Volume 10, Issue 2
Summer 2018
Pages 249-261

  • Receive Date 27 October 2017
  • Revise Date 24 February 2018
  • Accept Date 31 May 2018

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