The Effect of Aerobic Exercise in Carbon Black Particulates Air Pollution on TLR4 and TNF-α Gene Expression in Lung Tissue of Male Rats

Fashi, Mohammad; Agha-Alinejad, Hamid; Asilian Mahabadi, Hasan; Rezaee Seraji, Batool; Pak Rad, Behzad

doi:10.22059/jsb.2015.57472

The Effect of Aerobic Exercise in Carbon Black Particulates Air Pollution on TLR4 and TNF-α Gene Expression in Lung Tissue of Male Rats

Document Type : Research Paper

Authors

Mohammad Fashi ¹

Hamid Agha-Alinejad ²

Hasan Asilian Mahabadi ³

Batool Rezaee Seraji ⁴

Behzad Pak rad ⁴

¹ Assistant Professor, Faculty of Physical Education and Sport Sciences, Shahid Beheshti University, Tehran, Iran

² Associate Professor of Exercise Physiology, Department of Physical Education and Sports Sciences, Tarbiat Modares University, Tehran, Iran

³ Associate Professor of Occupational Health Engineering, Department of Environmental & Occupational Health, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

⁴ PhD in Exercise Physiology, Department of Physical Education and Sport Sciences, Tarbiat Modares University, Tehran, Iran

https://doi.org/10.22059/jsb.2015.57472

Abstract

The present study aimed at evaluating the effect of aerobic exercise in carbon black particulates air pollution on TLR4 and TNF-α gene expression in male rats. 24 male Wistar rats (mean body mass 279.29+26.97 gr) were randomly divided into 4 groups: A) control, B) aerobic exercise, C) pollution, D) exercise+pollution. A particulates injector and Falounak chamber were used to expose the rats to carbon black particulates with a diameter˂10 micron. Aerobic exercise protocol was performed in each group with 50% maximum speed for 4 weeks. The subjects were killed 24 hours after the last session and exposure to carbon black particulates. The gene expression of TLR4, and TNF-α were analyzed in the lung tissue by Real time – Pcr technique. In order to determine the significant differences among the groups, multiple ANOVA, LSD post hoc and non-parametric Friedman tests were used. Aerobic exercise reduced the gene expression of TLR4, and TNF-α in rats exposed to carbon black particulates. Also, the exercise significantly reduced body mass in those groups exposed to carbon black particulates. 4 weeks of aerobic exercise in carbon black particulates air pollution balanced inflammatory markers of TLR4, TNF-α and body mass. It seems that these changes are partly associated with a reduction in the risk of pulmonary diseases.

Keywords

aerobic exercise

carbon black

inflammation

lung tissue

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