The Concurrent Effect of Eccentric Resistance Training and Blood Flow Occlusion on STAT3 and MyF5 Gene Expressions Affecting the Activation of Satellite Cells Growth in Non-Athletes

Tabibi, Mohammad Ali; Mosavian, Azam; Gaeini, Abasali; Ghara Khanlou, Reza; Nuri, Reza; Kordi, Mohamadreza

doi:10.22059/jsb.2020.227486.1147

Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license

Authors

¹ PhD Student of Exercise Physiology, Kish International Campus, University of Tehran, Kish, Iran

² Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran

³ Professor, Department of Physical Education, Tarbiat Modares University, Tehran, Iran

⁴ . Assistant Professor, Department of Sport Sciences, Kish International Campus, University of Tehran, Kish, Iran

⁵ Associate Professor, Department of Sport Sciences, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran

https://doi.org/10.22059/jsb.2020.227486.1147

Abstract

The aim of the present study was to investigate the effect of resistance training and blood flow occlusion on gene expression of signal transfer agent and activator of transcription (STAT3) and muscle-forming agent 5 (Myf5) in non-athlete active young subjects. 20 men (age range 25 ± 5 years) were randomly divided into two groups (ach group 10 subjects): eccentric resistance training with and without blood flow restriction (BFR). Using isokinetic set, eccentric resistance training protocol (ECC RET) including about 70 repetitions of knee extensor muscles was performed. Occlusion was created using pneumatic pressure device. After local anesthesia with lidocaine 1% and norepinephrine, blood samples were collected from active vastus lateralis 48 hours before and 24 hours after the training. STAT3 and MyF5 were measured by immunohistochemistry. Gene expression of STAT3 in BFR group in the posttest was 3.846 times higher than the pretest (P=0.001) which showed a significant difference. Gene expression of MyF5 in BFR group in the posttest was 3.479 times higher than the pretest (P=0.005) which showed a significant difference. Eccentric resistance training with blood flow occlusion may maintain and improve muscle mass by increasing the gene expression of STAT3 and MyF5.

Keywords

Main Subjects

Exercise Physiology

References

1. Fry CS, Glynn EL, Drummond MJ, Timmerman KL, Fujita S, Abe T, et al. Blood flow restriction exercise stimulates mTORC1 signaling and muscle protein synthesis in older men. J Appl Physiol. 2010 May;108(5):1199–209.

2. Takarada Y, Nakamura Y, Aruga S, Onda T, Miyazaki S, Ishii N. Rapid increase in plasma growth hormone after low-intensity resistance exercise with vascular occlusion. J Appl Physiol. 2000;88(1):61–5.

3. Fujita S, Abe T, Drummond MJ, Cadenas JG, Dreyer HC, Sato Y, et al. Blood flow restriction during low-intensity resistance exercise increases S6K1 phosphorylation and muscle protein synthesis. J Appl Physiol [Internet]. 2007 [cited 2020 Sep 16];103(3):903–10. Available from: http://www.

4. Pedersen BK. Muscles and their myokines. J Exp Biol [Internet]. 2011 Jan 15 [cited 2020 Sep 16];214(2):337–46. Available from: https://jeb.biologists.org/content/214/2/337

5. Pedersen BK, Åkerström TCA, Nielsen AR, Fischer CP. Role of myokines in exercise and metabolism. Vol. 103, Journal of Applied Physiology. 2007. p. 1093–8.

6. science LQ-J of animal, 2008 undefined. Interleukin-15: A muscle-derived cytokine regulating fat-to-lean body composition,. academic.oup.com [Internet]. [cited 2020 Sep 16]; Available from: https://academic.oup.com/jas/article-abstract/86/suppl_14/E75/ 4789854

7. Nielsen JL, Aagaard P, Bech RD, Nygaard T, Hvid LG, Wernbom M, et al. Proliferation of myogenic stem cells in human skeletal muscle in response to low-load resistance training with blood flow restriction. J Physiol. 2012 Sep;590(17):4351–61.

8. Serrano A, Baeza-Raja B, Perdiguero E, metabolism MJ-C, 2008 undefined. Interleukin-6 is an essential regulator of satellite cell-mediated skeletal muscle hypertrophy. Elsevier [Internet]. [cited 2020 Sep 16]; Available from: https://www.sciencedirect.com/science/ article/pii/S155041310700366X

9. HATHER BM, TESCH PA, BUCHANAN P, DUDLEY GA. Influence of eccentric actions on skeletal muscle adaptations to resistance training. Acta Physiol Scand. 1991;143(2):177–85.

10. Sorichter S, Mair J, Koller A, Gebert W, Rama D, Calzolari C, et al. Skeletal troponin I as a marker of exercise-induced muscle damage. J Appl Physiol. 1997;83(4):1076–82.

11. Roig M, O’brien K, Shadgan B, Reid D. The effects of eccentric versus concentric resistance training on muscle strength and mass in healthy adults: A systematic review with meta-analysis Top Cited Articles in Medicine View project Optical Diagnosis of Acute Scrotum View project. bjsm.bmj.com [Internet]. 2014 [cited 2020 Sep 16]; Available from: http://bjsm.bmj.com/cgi/content/full/43/8/556#BIBL

12. McHugh MP, Connolly DAJ, Eston RG, Gleim GW. Exercise-induced muscle damage and potential mechanisms for the repeated bout effect. Vol. 27, Sports Medicine. 1999. p. 157–70.

13. Nielsen AR, Pedersen BK. The biological roles of exercise-induced cytokines: IL-6, IL-8, and IL-15. Vol. 32, Applied Physiology, Nutrition and Metabolism. 2007. p. 833–9.

14. Paulsen G, Mikkelsen UR, Peake J. Leucocytes, cytokines and satellite cells: What role do they play in muscle damage and regeneration following eccentric exercise? [Internet]. researchgate.net. [cited 2020 Sep 16]. Available from: https://www.researchgate.net/ publication/230643819

15. Patterson SD, Leggate M, Nimmo MA, Ferguson RA. Circulating hormone and cytokine response to low-load resistance training with blood flow restriction in older men. Eur J Appl Physiol. 2013;113(3):713–9.

16. Ciciliot S, Schiaffino S. Regeneration of Mammalian Skeletal Muscle: Basic Mechanisms and Clinical Implications [Internet]. Vol. 16, Current Pharmaceutical Design. 2010 [cited 2020 Sep 16]. Available from: https://www.ingentaconnect.com/content/ben/cpd/2010 /00000016/00000008/art00002

17. Ochi E, Nakazato K, & NI-TJ of S, 2011 undefined. Muscular hypertrophy and changes in cytokine production after eccentric training in the rat skeletal muscle. cdn.journals.lww.com [Internet]. [cited 2020 Sep 16]; Available from: https://cdn. journals.lww.com/nsca-jscr/Fulltext/2011/08000/Muscular_Hypertrophy_and_Changes_in_Cytokine.28.aspx

18. Tatsumi R, Liu X, Pulido A, Morales M, Sakata T, Dial S, et al. Satellite cell activation in stretched skeletal muscle and the role of nitric oxide and hepatocyte growth factor. Am J Physiol - Cell Physiol. 2006 Jun;290(6).

19. Umbel JD, Hoffman RL, Dearth DJ, Chleboun GS, Manini TM, Clark BC. Delayed-onset muscle soreness induced by low-load blood flow-restricted exercise. Eur J Appl Physiol. 2009 Dec;107(6):687–95.

20. Wernbom M, Apro W, Paulsen G, Nilsen TS, Blomstrand E, Raastad T. Acute low-load resistance exercise with and without blood flow restriction increased protein signalling and number of satellite cells in human skeletal muscle. Eur J Appl Physiol. 2013 Dec;113(12):2953–65.

21. Guadagnin E, Mázala D, Chen Y-W. Molecular Sciences Review STAT3 in Skeletal Muscle Function and Disorders. mdpi.com [Internet]. 2018 [cited 2020 Sep 16]; Available from: www.mdpi.com/journal/ijms

22. Gopinath SD. Inhibition of stat3 signaling ameliorates atrophy of the soleus muscles in mice lacking the vitamin D receptor. Skelet Muscle. 2017 Jan 25;7(1).

23. Zimmers T, Fishel M, developmental AB-S in cell &, 2016 undefined. STAT3 in the systemic inflammation of cancer cachexia. Elsevier [Internet]. [cited 2020 Sep 16]; Available from: https://www.sciencedirect.com/science/article/pii/S1084952116300519

24. Tierney M, Aydogdu T, Sala D, Malecova B, medicine SG-N, 2014 undefined. STAT3 signaling controls satellite cell expansion and skeletal muscle repair. nature.com [Internet]. [cited 2020 Sep 16]; Available from: https://www.nature.com/nm/journal/v20/n10/abs /nm.3656.html

Journal of Sport Biosciences

The Concurrent Effect of Eccentric Resistance Training and Blood Flow Occlusion on STAT3 and MyF5 Gene Expressions Affecting the Activation of Satellite Cells Growth in Non-Athletes

References

References

Volume 12, Issue 2
September 2020
Pages 143-154

The Concurrent Effect of Eccentric Resistance Training and Blood Flow Occlusion on STAT3 and MyF5 Gene Expressions Affecting the Activation of Satellite Cells Growth in Non-Athletes

References

References

Volume 12, Issue 2September 2020Pages 143-154

Volume 12, Issue 2
September 2020
Pages 143-154