The Effect of 8 Weeks of high-Intensity Interval Training on Klotho, PLP and TNF-α Cerebellum Tissue  Mice

Farahmand, Fataneh; Nourshahi, Maryam; Soleimani, Maryam; Rajabi, Hamed; Power, Kevin

doi:10.22059/jsb.2020.292695.1367

Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license

Authors

¹ Department of Biological Sciences in Sport and Health, Faculty of Sports Sciences and Health, Shahid Beheshti University, Tehran, Iran

² Department of Medical Basic Sciences, University of Social Welfare and Rehabilitation Sciences Tehran, Iran,

³ Department of Exercise Physiology, Sport Science Faculty, Kharazmi University, Tehran, Iran

⁴ Department of Exercise Physiology, Sport Science Faculty, Kharazmi University, Tehran, Iran,

⁵ School of Human Kinetics and Recreation, Memorial University of Newfoundland, St. John’s, NL, Canada

https://doi.org/10.22059/jsb.2020.292695.1367

Abstract

Nowadays, many studies have shown that exercise improves brain health and performance and may also increase neurological pathways in the brain. The aim of the present study was to investigate the effect of 8 weeks of high intensity interval training (HIIT) on Klotho, PLP and TNF-α concentrations in mice. Female mice were randomly divided to exercise (EX) or control (Con) groups. In the EX group, animals performed 8-week of HIIT. Mice initially ran at 6 m/min for 3 min at 0% grade, with the speed progressively increasing by 3 m/min every 3 min until exhaustion - when the mice were unable to maintain the required running speed. The maximal speed obtained was then used to calculate the individualized running speed for mice in the EX group. HIIT involved treadmill running 5 days per week for the final 6 weeks. Klotho, PLP and TNF-α expressions were studied using Western blot. Mice in the EX group had a significant increase in klotho and PLP concentrations compared to the control group (p≤0.01 and p≤0.05, respectively). Also, in the exercise groups mice had a significant decrease in TNF-α concentrations in compared to the control group (p≤0.05). Our data demonstrate that HIIT increased klotho and PLP and deceased neuroinflammatory cytokines. These proteins are associated with increasing myelination and protect the central nerve system against neurodegenerative diseases.

Keywords

References

1.White LJ, Castellano V. Exercise and brain health—implications for multiple sclerosis. Sports medicine. 2008;38(2):91-100.

2.Pavlatou M, Remaley A, Gold P. Klotho: a humeral mediator in CSF and plasma that influences longevity and susceptibility to multiple complex disorders, including depression. Translational psychiatry. 2016;6(8):e876.

3.Cararo-Lopes MM, Mazucanti CHY, Scavone C, Kawamoto EM, Berwick DC. The relevance of α-KLOTHO to the central nervous system: some key questions. Ageing research reviews. 2017;36:137-48.

4.Zeldich E, Chen C-D, Avila R, Medicetty S, Abraham CR. The anti-aging protein Klotho enhances remyelination following cuprizone-induced demyelination. Journal of Molecular Neuroscience. 2015;57(2):185-96.

5.Mazucanti CH, Kawamoto EM, Mattson MP, Scavone C, Camandola S. Activity-dependent neuronal Klotho enhances astrocytic aerobic glycolysis. Journal of Cerebral Blood Flow & Metabolism. 2019;39(8):1544-56.

6.Ahmadi M, Aleagha MSE, Harirchian MH, Yarani R, Tavakoli F, Siroos B. Multiple sdlerosis influences on the augmentation of serum Klotho concentration. Journal of the neurological sciences. 2016;362:69-72.

7.Aleagha MSE, Harirchian MH, Lavasani S, Javan M, Allameh AJJoMN. Differential expression of klotho in the brain and spinal cord is associated with total antioxidant capacity in mice with experimental autoimmune encephalomyelitis. 2018;64(4):543-50.

8.Maekawa Y, Ishikawa K, Yasuda O, Oguro R, Hanasaki H, Kida I, et al. Klotho suppresses TNF-α-induced expression of adhesion molecules in the endothelium and attenuates NF-κB activation. Endocrine. 2009;35(3):341-6.

9.Wolf I, Levanon-Cohen S, Bose S, Ligumsky H, Sredni B, Kanety H, et al. Klotho: a tumor suppressor and a modulator of the IGF-1 and FGF pathways in human breast cancer. Oncogene. 2008;27(56):7094.

10.Zeldich E, Chen C-D, Colvin TA, Bove-Fenderson EA, Liang J, Zhou TBT, et al. The neuroprotective effect of Klotho is mediated via regulation of members of the redox system. 2014;289(35):24700-15.

11.Chen C-D, Sloane JA, Li H, Aytan N, Giannaris EL, Zeldich E, et al. The antiaging protein klotho enhances oligodendrocyte maturation and myelination of the CNS. Journal of Neuroscience. 2013;33(5):1927-39.

12.Baron W, Ozgen H, Klunder B, de Jonge JC, Nomden A, Plat A, et al. The major myelin-resident protein PLP is transported to myelin membranes via a transcytotic mechanism: involvement of sulfatide. Molecular and cellular biology. 2015;35(1):288-302.

13.Simons M, Krämer E-M, Thiele C, Stoffel W, Trotter J. Assembly of myelin by association of proteolipid protein with cholesterol-and galactosylceramide-rich membrane domains. J Cell Biol. 2000;151(1):143-54.

14.Mostafidi E, Moeen A, Nasri H, Hagjo AG, Ardalan M. Serum Klotho Levels in Trained Athletes. Nephro-urology monthly. 2016;8(1).

15.Ji N, Luan J, Hu F, Zhao Y, Lv B, Wang W, et al. Aerobic exercise‑stimulated Klotho upregulation extends life span by attenuating the excess production of reactive oxygen species in the brain and kidney. Experimental and therapeutic medicine. 2018;16(4):3511-7.

16.Bernardes D, Brambilla R, Bracchi‐Ricard V, Karmally S, Dellarole A, Carvalho‐Tavares J, et al. Prior regular exercise improves clinical outcome and reduces demyelination and axonal injury in experimental autoimmune encephalomyelitis. Journal of neurochemistry. 2016;136:63-73.

17.Bernardes D, Oliveira-Lima OC, da Silva TV, Faraco CCF, Leite HR, Juliano MA, et al. Differential brain and spinal cord cytokine and BDNF levels in experimental autoimmune encephalomyelitis are modulated by prior and regular exercise. Journal of neuroimmunology. 2013;264(1-2):24-34.

18.Bartalucci A, Ferrucci M, Fulceri F, Lazzeri G, Lenzi P, Toti L, et al. High-intensity exercise training produces morphological and biochemical changes in adrenal gland of mice. 2012.

19.Afzalpour ME, Chadorneshin HT, Foadoddini M, Eivari HA. Comparing interval and continuous exercise training regimens on neurotrophic factors in rat brain. Physiology & behavior. 2015;147:78-83.

20.Matsubara T, Miyaki A, Akazawa N, Choi Y, Ra S-G, Tanahashi K, et al. Aerobic exercise training increases plasma klotho levels and reduces arterial stiffiness in postmenopausal women. Americal Journal of Ohysiology-Heart and Circulatory Physiology. 2013;306(3):H348-H55.

21.Saghiv M, Sherve C, Ben-Sira D, Sagiv M, Goldhammer E. Aerobic Training Effect on Blood S-Klotho Levels in Coronary Artery Disease Patients. Journal of Clinical & Experimental Cardiology. 2016;7:1-4.

22.Santos-Dias A, MacKenzie B, Oliveira-Junior MC, Moyses RM, Consolim-Colombo FM, Vieira RP. Longevity protein klotho is induced by a single bout of exercise. Br J Sports Med. 2017;51(6):549-50.

23.Saghiv M, Sherve C, Ben-Sira D, Sagiv M, Goldhammer E. Aerobic training effect on blood s-Klotho levels in coronary artery disease patients. J Clin Exp Cardiol. 2016;7(8):464.

24.Saghiv M, Sherve C, Goldhammer E, Ben-Sira D, Sagiv M. Long Lasting Chronic Resistive Training Effects on Circulating S-Klotho and IGF-1. Archives of Clinical and Biomedical Research. 2017;1:69-75.

25.Yoon H, Kleven A, Paulsen A, Kleppe L, Wu J, Ying Z, et al. Interplay between exercise and dietary fat modulates myelinogenesis in the central nervous system. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease. 2016;1862(4):545-55.

26.Moein A, Nikbakht H, Ghazalian F. The effect of aerobic training on Klotho protein and a selected of inflammatory indices in kidney of Male Rats. Research in Medicine. 2017;41(3):175-82.

27.Buendía P, Ramíez R, Aljama P, Carracedo J. Chapter Five-Klotho Prevents Translocation of NFκB. Vitamins & Hormones. 2016;101:119-50.

28.Dalgas U, Stenager E. Exercise and disease progression in multiple sclerosis: can exercise slow down the progression of multiple sclerosis? Therapeutic advances in neurological disorders. 2012;5(2):81-95.

29.Ang E, Gomez-Pinilla F. Potential therapeutic effects of exercise to the brain. Current medicinal chemistry. 2007;14(24):2564-71.

30.Ding Y-H, Mrizek M, Lai Q, Wu Y, Reyes R, Li J, et al. Exercise preconditioning reduces brain damage and inhibits TNF-α receptor expression after hypoxia/reoxygenation: an in vivo and in vitro study. Current neurovascular research. 2006;3(4):263-71.

Journal of Sport Biosciences

The Effect of 8 Weeks of high-Intensity Interval Training on Klotho, PLP and TNF-α Cerebellum Tissue Mice

References

References

Volume 12, Issue 1
June 2020
Pages 67-78

The Effect of 8 Weeks of high-Intensity Interval Training on Klotho, PLP and TNF-α Cerebellum Tissue Mice

References

References

Volume 12, Issue 1June 2020Pages 67-78

Volume 12, Issue 1
June 2020
Pages 67-78