The Effect of Aerobic Exercise and Calorie Restriction on Intramuscular GLUT4 and DAG Levels in Obese Male Rats

Jorbonian, Aboozar; Mohebbi, Hamid

doi:10.22059/jsb.2018.66075

Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license

Authors

Aboozar Jorbonian ¹
Hamid Mohebbi ²

¹ PhD Student of Exercise Physiology, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Iran

² Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Iran

https://doi.org/10.22059/jsb.2018.66075

Abstract

Insulin resistance induced by a high fat diet has been associated with intramuscular lipid metabolites. The aim of this study was to investigate the effect of aerobic exercise, diet restriction and their combination on insulin resistance through intramuscular diacylglycerol changes in obese male rats. Sample consisted of 56 male Wistar rats (mean weight: 194.5±12.6 g). 48 rats had a high-fat diet for 18 weeks and 8 rats had normal diet. Then, high-fat diet rats were divided into control (C), aerobic Exercise (E), caloric restriction (CR), and a combination (aerobic Exercise + caloric restriction (E+C) groups. E group ran on a treadmill at 28 m. per minute for 10 weeks. In CR group, 25% of their daily caloric ingestion reduced and E+C group had aerobic exercise and caloric restriction every other day. The results indicated that rats gained weight after 18 weeks of high-fat diet (P<0.05). Also, there was a significant difference in muscle DAG and serum levels of insulin, glucose and insulin resistance index between aerobic exercise, calorie restriction and combination groups and high-fat group (P<0.05). PKC-θ levels decreased in negative energy balance groups (P>0.05). Also, there was a significant difference between E and E+C groups and high-fat diet group in GLUT4 levels (P>0.05). This study indicated that negative energy balance methods can decrease skeletal muscle DAG even along with a high-fat diet which is largely congruent with an improvement in insulin resistance.

Keywords

References

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Journal of Sport Biosciences

The Effect of Aerobic Exercise and Calorie Restriction on Intramuscular GLUT4 and DAG Levels in Obese Male Rats

References

References

Volume 9, Issue 4
May 2016
Pages 501-514

The Effect of Aerobic Exercise and Calorie Restriction on Intramuscular GLUT4 and DAG Levels in Obese Male Rats

References

References

Volume 9, Issue 4May 2016Pages 501-514

Volume 9, Issue 4
May 2016
Pages 501-514