Introduction: Type 2 diabetes is associated with chronic inflammation. While exercise and supplements have been shown to influence inflammation, the combined effects of aerobic exercise and grape seed extract on inflammatory markers in middle-aged women with T2D remain unknown. This study examined this combined intervention.

Methods This study is a semi-experimental research. The statistical population consists of inactive obese women with type 2 diabetes in Kermanshah province. The participants Based on the inclusion criteria, 32 middle-aged women (45–57 years old) were chosen and randomly divided into four groups (8 participants per group): (1) grape seed extract supplement + exercise, (2) exercise + placebo, (3) placebo, and (4) grape seed extract supplement. Aerobic exercise was conducted for four weeks, with four sessions per week (a total of 20 sessions). Additionally, the participants consumed 263 mg of grape seed extract daily for four weeks. Data were analyzed using SPSS software through repeated measures analysis of variance.

Results: The results show that the values of insulin resistance (HOMA-IR), IL-6, cortisol, and prostaglandin in the exercise + supplement, exercise, and supplement groups decreased significantly compared to the control group (P = 0.0001). Additionally, the values of NO and IL-10 in the exercise + supplement, exercise, and supplement groups increased significantly compared to the control group (P = 0.0001).

Conclusion: A combined exercise and grape seed extract demonstrated significant benefits in reducing inflammation. Furthermore, this research indicated that the combination of exercise and grape seed extract improved the condition of individuals with type 2 diabetes.