University of Tehran Press Sport Biosciences 2008-9325 13 4 2021 12 22 Impact of Cycling Versus Sitting in Hot water and land on serum CXCL1 and IL-6 and insulin resistance in males with Metabolic Syndrome Impact of Cycling Versus Sitting in Hot water and land on serum CXCL1 and IL-6 and insulin resistance in males with Metabolic Syndrome 407 425 86248 10.22059/jsb.2021.322625.1465 FA Mohammad Taher Jahangiri Master of Science of Exercise Physiology, Department of Sport Sciences, Faculty of Education and Psychology, Azarbaijan Shahid Madani University, Tabriz, Iran. Roghiyeh Fakhrpour Assistant Professor of Exercise Physiology, Department of Sport Sciences, Faculty of Education and Psychology, Azarbaijan Shahid Madani University, Tabriz, Iran. Karim Azali Alamdari Associate Professor of Exercise Physiology, Azarbaijan Shahid Madani University, Tabriz, Iran 0000-0002-6134-1912 Journal Article 2021 04 25 Aim: exercise and hot water immersion is likely to more increase serum IL-6 and CXCL₁ as well as insulin resistance in patients with metabolic syndrome. However, no straight investigation has been done in this area. Therefore, the aim of study was to investigate effect of an acute cycling session while immersed in hot water versus land on serum IL-6 and CXCL₁ level and also insulin resistance in male patients with metabolic syndrome.Methodology: 15 males with metabolic syndrome (age: 58.4±4.17, BMI: 31.27±3.27) experienced four test sessions (with 48 hour intervals) including on cycling or inactive sitting in hot water (42 0°C) head out immersion or land conditions with a cross over design. Each of cycling sessions were included on 30 min of interval activity at 50% of MHR. Blood samples were taken before and 15 min post intervention. The variables were quantified using Elisa and Enzymatic methods and the data were analyzed by ANOVA for repeated measurements (time series method) at 0.05 statistical significance level. Results: Cycling increased serum CXCL1 only in the hot water, while IL-6 elevation was only noted during land exercise (P<0.05). However, insulin resistance was lowered in both hot water (p=0.002) and land exercise (p=0.001) sessions with no difference for this decline, between the two sessions(p=0.217). Conclusion: Cycling within the hot water compared to land, do not provide a remarkable advantage regarding the amount of induced changes in serum IL-6 and CXCL1 and even insulin resistance. However, more investigations remain to be done because of the lack of evidence and study limitations.  Aim: exercise and hot water immersion is likely to more increase serum IL-6 and CXCL₁ as well as insulin resistance in patients with metabolic syndrome. However, no straight investigation has been done in this area. Therefore, the aim of study was to investigate effect of an acute cycling session while immersed in hot water versus land on serum IL-6 and CXCL₁ level and also insulin resistance in male patients with metabolic syndrome.Methodology: 15 males with metabolic syndrome (age: 58.4±4.17, BMI: 31.27±3.27) experienced four test sessions (with 48 hour intervals) including on cycling or inactive sitting in hot water (42 0°C) head out immersion or land conditions with a cross over design. Each of cycling sessions were included on 30 min of interval activity at 50% of MHR. Blood samples were taken before and 15 min post intervention. The variables were quantified using Elisa and Enzymatic methods and the data were analyzed by ANOVA for repeated measurements (time series method) at 0.05 statistical significance level. Results: Cycling increased serum CXCL1 only in the hot water, while IL-6 elevation was only noted during land exercise (P<0.05). However, insulin resistance was lowered in both hot water (p=0.002) and land exercise (p=0.001) sessions with no difference for this decline, between the two sessions(p=0.217). Conclusion: Cycling within the hot water compared to land, do not provide a remarkable advantage regarding the amount of induced changes in serum IL-6 and CXCL1 and even insulin resistance. However, more investigations remain to be done because of the lack of evidence and study limitations.  https://jsb.ut.ac.ir/article_86248_a175cf87b6ee7112530e325cd480e67f.pdf