University of Tehran Press Sport Biosciences 2008-9325 11 1 2019 05 22 A Comparison of the Effect of 12 Weeks of Interval Training with High and Moderate Intensity on Perilipin 3 of Visceral Adipose Tissue and Insulin Resistance in Type 2 Diabetic Male Rats A Comparison of the Effect of 12 Weeks of Interval Training with High and Moderate Intensity on Perilipin 3 of Visceral Adipose Tissue and Insulin Resistance in Type 2 Diabetic Male Rats 35 48 71615 10.22059/jsb.2018.261519.1289 FA Kamilia Moghaddami PhD Student of Exercise Physiology, University Campus, Guilan University, Rasht, Iran Hamid Mohebbi Professor, Faculty of Physical Education and Sport Sciences, Guilan University, Rasht, Iran Elma Tabari PhD Student of Exercise Physiology, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Guilan University, Rasht, Iran Mehrsa Faridnia Professor, Faculty of Physical Education and Sport Sciences, Guilan University, Rasht, Iran Journal Article 2018 07 06 The aim of this study was to compare the effect of 12 weeks of interval training with high and moderate intensity on perilipin 3 (PLIN3) of visceral adipose tissue and insulin resistance in type 2 diabetic male rats. In this study, 24 male rats with type 2 diabetes were randomly divided into three groups: interval training with moderate intensity (MIIT), interval training with high intensity (HIIT) and control. Interval training (HIIT, MIIT) was applied for 12 weeks, 5 sessions per week. The MIIT protocol included 13 four-min. bouts of activity with intensity of 65-70% vo_2max and the HIIT protocol included 10 four-min. bouts of activity with intensity of 85-90% vo_2max with 2-minute active rest periods. Western Blot method was used to measure PLIN3 protein levels. ANOVA and Tukey's test were used for data analysis. The results indicated a significant decrease in PLIN3 in the HIIT and MIIT groups compared with the control group (<em>P</em>=0.001). However, there was no significant difference between the training groups (<em>P</em>=0.274). Also, both HIIT and MIIT protocols significantly decreased serum levels of glucose (<em>P</em>=0.001), while they had no significant effects on serum insulin and insulin resistance (<em>P</em>˃0.05). There was no significant difference between training groups in insulin, glucose and insulin resistance changes (<em>P</em>˃0.05). The findings of this study showed that both HIIT and MIIT protocols drastically reduced PLIN3 of visceral adipose and improved glucose metabolism in type 2 diabetic rats. Therefore, it seems that PLIN3 changes in visceral adipose are independent of the training intensity. The aim of this study was to compare the effect of 12 weeks of interval training with high and moderate intensity on perilipin 3 (PLIN3) of visceral adipose tissue and insulin resistance in type 2 diabetic male rats. In this study, 24 male rats with type 2 diabetes were randomly divided into three groups: interval training with moderate intensity (MIIT), interval training with high intensity (HIIT) and control. Interval training (HIIT, MIIT) was applied for 12 weeks, 5 sessions per week. The MIIT protocol included 13 four-min. bouts of activity with intensity of 65-70% vo_2max and the HIIT protocol included 10 four-min. bouts of activity with intensity of 85-90% vo_2max with 2-minute active rest periods. Western Blot method was used to measure PLIN3 protein levels. ANOVA and Tukey's test were used for data analysis. The results indicated a significant decrease in PLIN3 in the HIIT and MIIT groups compared with the control group (<em>P</em>=0.001). However, there was no significant difference between the training groups (<em>P</em>=0.274). Also, both HIIT and MIIT protocols significantly decreased serum levels of glucose (<em>P</em>=0.001), while they had no significant effects on serum insulin and insulin resistance (<em>P</em>˃0.05). There was no significant difference between training groups in insulin, glucose and insulin resistance changes (<em>P</em>˃0.05). The findings of this study showed that both HIIT and MIIT protocols drastically reduced PLIN3 of visceral adipose and improved glucose metabolism in type 2 diabetic rats. Therefore, it seems that PLIN3 changes in visceral adipose are independent of the training intensity. https://jsb.ut.ac.ir/article_71615_3b7ce5d325b87e53665443e5061ba127.pdf