University of Tehran Press Sport Biosciences 2008-9325 10 1 2018 05 22 Effect of Unsaturated-High-Fat-Diet and Aerobic Training Program on Serum levels of Adiponectin Isoforms, Insulin Resistance and Lipid Profile in Male obese Rats Effect of Unsaturated-High-Fat-Diet and Aerobic Training Program on Serum levels of Adiponectin Isoforms, Insulin Resistance and Lipid Profile in Male obese Rats 103 118 67026 10.22059/jsb.2018.250623.1238 FA Payam Saidie . Assistant Professor, Guilan University, Rasht, Iran 0000-0001-7325-9484 Hamid Mohebbi . Professor, Guilan University, Rasht, Iran 0000-0003-1524-9530 Farhad Rahmani Nia . Professor, Guilan University, Rasht, Iran Fahime Mohammad Ghasemi Histology Department, Medical science University, Guilan university, Rasht, Iran Journal Article 2018 01 19 The aim of present study was to investigate the effect of 10-week aerobic training and unsaturated-high-fat-diet on adiponectin serum in obese male rats. Blood samples were collected of forty-eight wistar male rats (average weight 195±5g, age 8 weeks) at 1st, 18th and 28th weeks after overnight fasting. After first sampling, remaining 40 rats were randomly divided into control (n=16) and high-fat-diet (39% of energy content from unsaturated-fat) (n=24). High-fat-diet group randomly divided in to exercise training and high-fat-diet subgroups after 18 weeks. Exercise training group ran for 10 weeks (5d/wk) with intensity equivalent to 70 to 75% of vo2max. Serum levels of total and HMW adiponectin as well as lipid profile and glycemic variables were measured. The results of this study indicate that in comparison to unsaturated-high-fat-diet, aerobic training in unsaturated-high-fat-diet induced obese male rats, increased serum concentration of total adiponectin significantly (P> 0/01). Also insulin resistance index and serum concentrations of insulin, glucose, total cholesterol, LDL-C and TG were decreased in aerobic exercise compare to high-fat-diet group (P> 0/05). This study indicates that aerobic training increase serum concentrations of total adiponectin in obese male rats even with simultaneous consumption of unsaturated-high-fat-diet and cause improvement in lipid and metabolic profiles. The aim of present study was to investigate the effect of 10-week aerobic training and unsaturated-high-fat-diet on adiponectin serum in obese male rats. Blood samples were collected of forty-eight wistar male rats (average weight 195±5g, age 8 weeks) at 1st, 18th and 28th weeks after overnight fasting. After first sampling, remaining 40 rats were randomly divided into control (n=16) and high-fat-diet (39% of energy content from unsaturated-fat) (n=24). High-fat-diet group randomly divided in to exercise training and high-fat-diet subgroups after 18 weeks. Exercise training group ran for 10 weeks (5d/wk) with intensity equivalent to 70 to 75% of vo2max. Serum levels of total and HMW adiponectin as well as lipid profile and glycemic variables were measured. The results of this study indicate that in comparison to unsaturated-high-fat-diet, aerobic training in unsaturated-high-fat-diet induced obese male rats, increased serum concentration of total adiponectin significantly (P> 0/01). Also insulin resistance index and serum concentrations of insulin, glucose, total cholesterol, LDL-C and TG were decreased in aerobic exercise compare to high-fat-diet group (P> 0/05). This study indicates that aerobic training increase serum concentrations of total adiponectin in obese male rats even with simultaneous consumption of unsaturated-high-fat-diet and cause improvement in lipid and metabolic profiles. https://jsb.ut.ac.ir/article_67026_0bee2582287092193eca43328a208286.pdf