University of Tehran Press Sport Biosciences 2008-9325 7 3 2015 09 23 The Effects of 8 Weeks of Endurance Training on α -1A Protein of pre-Synaptic P-Q-type Calcium Channels in Fast and slow Muscles of Rats The Effects of 8 Weeks of Endurance Training on α -1A Protein of pre-Synaptic P-Q-type Calcium Channels in Fast and slow Muscles of Rats 363 374 56252 10.22059/jsb.2015.56252 FA Ali Gorzi Assistant Professor, Department of Physical Education and Sport Sciences, University of Zanjan, Zanjan, Iran Hamid Rajabi Associate Professor, Faculty of Physical Education and Sport Sciences, University of Kharazmi, Tehran, Iran 0000-0002-3276-1598 Reza Ghara Khanlou Associate Professor, Department of Physical Education and Sport Sciences, Tarbiat Modares University, Tehran, Iran Journal Article 2013 11 09 The purpose of this study was to investigate the effects of 8 weeks of endurance training (ET) on ? -1A protein of pre-synaptic P-Q-type Calcium Channels in FHL and Soleus muscles of rats. 16 male wistar rats provided from razi institute, were randomly divided to 2 groups (Control-Sham; n=8 and Endurance Training; n= 8). Training group performed 8 weeks (5 session/week) of endurance training on animal treadmill with 10 m/min for 30 min in the first week which was gradually increased to 30 m/min for 60 min(equal to 70-80% of Vo2max) in the last week. 48 h after last session of training, FHL and Sol muscles were removed under sterilized situation by cutting on posterio-lateral side of hind limb. Measuring ?-1A protein with Western Blotting and independent t test showed that the amount of this protein insignificantly (P=0.177) increased in soleus muscles of ET group (soleus: 14.82±87.44 vs. Control: 20.20±72.57 (equal to 0/20 percent) and FHL: 7.26±70.83 vs. Control: 6.28±70.01- p=0/839). This insignificant increase in ? -1A protein in soleus muscles, may indicate to the responsiveness of pre-synaptic P-Q-type calcium channels of muscles following endurance training for improving Ach release from pre-synaptic terminal, noted in NMJ adaptations. In conclusion, it might be expressed that endurance training could be a main factor for the function of ?-1A protein in muscles and this case should be studied in future investigations with higher volume and intensities. The purpose of this study was to investigate the effects of 8 weeks of endurance training (ET) on ? -1A protein of pre-synaptic P-Q-type Calcium Channels in FHL and Soleus muscles of rats. 16 male wistar rats provided from razi institute, were randomly divided to 2 groups (Control-Sham; n=8 and Endurance Training; n= 8). Training group performed 8 weeks (5 session/week) of endurance training on animal treadmill with 10 m/min for 30 min in the first week which was gradually increased to 30 m/min for 60 min(equal to 70-80% of Vo2max) in the last week. 48 h after last session of training, FHL and Sol muscles were removed under sterilized situation by cutting on posterio-lateral side of hind limb. Measuring ?-1A protein with Western Blotting and independent t test showed that the amount of this protein insignificantly (P=0.177) increased in soleus muscles of ET group (soleus: 14.82±87.44 vs. Control: 20.20±72.57 (equal to 0/20 percent) and FHL: 7.26±70.83 vs. Control: 6.28±70.01- p=0/839). This insignificant increase in ? -1A protein in soleus muscles, may indicate to the responsiveness of pre-synaptic P-Q-type calcium channels of muscles following endurance training for improving Ach release from pre-synaptic terminal, noted in NMJ adaptations. In conclusion, it might be expressed that endurance training could be a main factor for the function of ?-1A protein in muscles and this case should be studied in future investigations with higher volume and intensities. https://jsb.ut.ac.ir/article_56252_8665b0699e891b7610e00f4294d709cd.pdf