University of Tehran Journal of Sport Biosciences 2008-9325 6 4 2015 01 21 The Effect of Resistance Exercise on MyoD Expression in Slow and Fast Muscles of Wistar Rats The Effect of Resistance Exercise on MyoD Expression in Slow and Fast Muscles of Wistar Rats 435 449 53217 10.22059/jsb.2015.53217 FA Mohammad Fathi Assistant Professor of Exercise Physiology, Physical Education Department, Humanities Faculty, Lorestan University, Khoramabad, Iran Reza Gharakhanlou Associate Professor of Exercise Physiology, Physical Education Department, Humanities Faculty, Tarbiat Modares University, Tehran, Iran Masoud Solimani Associate Professor, Hematology Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Hamid Rajabi Associate Professor of Exercise Physiology, Faculty of Physical Education and Sports Sciences, Kharazmi University, Karaj, Iran 0000-0002-3276-1598 Raziyeh Rezaei Ph.D. Student of Exercise Physiology, Shahid Chamran University, Ahvaz, Iran Journal Article 2013 12 10 Abstract<br />Background and Objective: satellite cells are muscle stem cells that enter cycle cells due to myotrauma. Among activation and proliferation indices of these cells is increase of myoD gene expression. But these are contradiction in response of this gene to resistance exercise in fast and slow fibers. Thus, the aim of this study was to evaluate the effect of a single bout of resistance exercise on myoD gene expression in fast and slow skeletal muscle in Wistar male rats.<br />Materials and Methods: 15 rats prepared from Pasteur Institute and housed under natural conditions (temperature, light/dark (12-h) cycle, with ad Libitum access to food and water). The rats randomly divided to two groups Resistance exercise (n=10) and control (n=5); the Resistance exercise group performed a session resistance exercise. three and six hours following, the rats were anaesthetized and killed, than to determine myoD gene expression rate the soleus and Extensor digitorum longus (EDL) muscles were removed, to determine of expression rate, the Quantitative Real time RT-PCR was used. Data were analyzed by t test.<br />Results: the Resistance exercise increased myoD gene expression 2.36 flod (no significantly) in EDL muscle, the SOL myoD gene expression no changed significantly. <br />Conclusion: myoD gene in fast-twitch muscle than in slow-twitch muscle is more and faster affected by resistance exercise. Abstract<br />Background and Objective: satellite cells are muscle stem cells that enter cycle cells due to myotrauma. Among activation and proliferation indices of these cells is increase of myoD gene expression. But these are contradiction in response of this gene to resistance exercise in fast and slow fibers. Thus, the aim of this study was to evaluate the effect of a single bout of resistance exercise on myoD gene expression in fast and slow skeletal muscle in Wistar male rats.<br />Materials and Methods: 15 rats prepared from Pasteur Institute and housed under natural conditions (temperature, light/dark (12-h) cycle, with ad Libitum access to food and water). The rats randomly divided to two groups Resistance exercise (n=10) and control (n=5); the Resistance exercise group performed a session resistance exercise. three and six hours following, the rats were anaesthetized and killed, than to determine myoD gene expression rate the soleus and Extensor digitorum longus (EDL) muscles were removed, to determine of expression rate, the Quantitative Real time RT-PCR was used. Data were analyzed by t test.<br />Results: the Resistance exercise increased myoD gene expression 2.36 flod (no significantly) in EDL muscle, the SOL myoD gene expression no changed significantly. <br />Conclusion: myoD gene in fast-twitch muscle than in slow-twitch muscle is more and faster affected by resistance exercise. https://jsb.ut.ac.ir/article_53217_8dc8533d83db4c765e8268afd8b4d3eb.pdf